Tribolium methods and resources

Please add your favorite protocol to this page by sending me a PDF-file (preferentially), or a RTF-file. Or tell me the URL of the respective file on your server ...

You may also want to check our articles in "Cold Spring Harbor Protocols" from 2009 (search PubMed for: Tribolium AND "Cold Spring Harb Protoc."[Journal])

Histology, in situ stainings

• Fixation and devitellinization of embryos for stainings
• two-colour in situ hybridization
• RNA probes for in situ hybridization
• Fuchsin-staining, benz mix embedding
• Antibody staining of non-devitellinized embryos

Classical genetics

• working with beetles (link to Beeman lab)
• genetic maps of Tribolium (link to Beeman lab)
• visible mutants in Tribolium (link to Beeman lab)
• working with embryonic lethals: Berghammer et al + Supplement
  

RNAi, morpholino knock down

• parental RNAi
• morpholino oligos

Transgenics

• germline transformation

Genomics

• molecular markers
• BAC library